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Sirard Marc-André

Membre chercheur régulier

Professeur titulaire
Université Laval
Directeur du Centre de Recherche en Reproduction, Développement et Santé Intergénérationnelle (CRDSI)

Thèmes de recherche

Thème 5 - Autonomie, prospérité et accessibilité alimentaire

Mots clés

métabolisme

épigénétique

reproduction

cycle de vie

étudiants gradués

Lima
Camila

(Directeur)

post-doc,
Ulaval

Projet: épigénétique embryonnaire

Wu
Chongyang

(Directeur)

doctorat,
Ulaval

Projet: épigénétique gamètes mâle

Zhang
Ying

(Directeur)

doctorat,
Ulaval

Projet: épugénétique gamete mâle

SHi
Meihog

(Directeur)

doctorat,
Ulaval

Projet: programmation métabolique de la mère

Lafontaine
Simon

(Directeur)

doctorat,
Ulaval

Projet: épigénétique embryonnaire et post natal

Robichaud
Julie-Pierre

(Directeur)

maitrise,
ULaval

Projet: metabolisme embryonnaire

Arjoune
Asma

(Directeur)

doctorat,
Ulaval

Projet: melatonine et fonction ovarienne

Dos Santos
Erika

(Directeur)

post doctorat,
Ulaval

Projet: metabolism embryonnaire

Wang
Mengqi

(Directeur)

doctorat,
Ulaval

Projet: épigénétique de la glande mammaire

Boulet
Martine

(Directeur)

maitrise,
Ulaval

Projet: metabolisme maternel et reproduction

Coordonnées


  • Université Laval

Faculté des sciences de l'agriculture et de l'alimentation
Département des sciences animales

Pavillon des Services - Local 2732
2440 boulevard Hochelaga
Québec (Québec) G1V 0A6
Canada

418-656-2131 - poste407359

Liens et réseaux sociaux

Expertises


Les intérêts de recherche du Dr Marc- André Sirard portent sur la compréhension de la fonction ovarienne, la compréhension du développement folliculaire, l’analyse de l’effet ovarien sur la qualité de l’ovule, l’analyse de l’environnement maternel sur la fonction ovarienne et la qualité des ovules chez les bovins (modèle) et chez l’humain et les études épigénétique de l’ovaire, l’ovule et de l’embryon.

Formation


Postdoctorat - Reproduction - University of Wisconsin

Doctorat - Médecine expérimentale - Université Laval

Doctorat - Médecine vétérinaire - Université de Montréal

En savoir plus


324: Shi M, SIRARD MA. Effects of NEFAs during IVM on pig embryos from granulosa cell-cocultured oocytes. Mol Reprod Dev. 2021 Nov 28. doi: 10.1002/mrd.23548.Epub ahead of print. PMID: 34841613.

323: Hallberg I, Persson S, Olovsson M, SIRARD MA, Damdimopoulou P, Rüegg J, Sjunnesson YCB. Perfluorooctane sulfonate (PFOS) exposure of bovine oocytes affects early embryonic development at human-relevant levels in an in vitro model. Toxicology. 2021 Nov 8:153028. doi: 10.1016/j.tox.2021.153028. Epub ahead of print. PMID: 34762985.

322: Shi M, SIRARD MA. Cocultured porcine granulosa cells respond to excess non-esterified fatty acids during in vitro maturation. J Ovarian Res. 2021 Oct 28;14(1):142. doi: 10.1186/s13048-021-00904-y. PMID: 34711256; PMCID:PMC8554973.

321: Rabaglino MB, Bojsen-Møller Secher J, SIRARD MA, Hyttel P, Kadarmideen HN.

Epigenomic and transcriptomic analyses reveal early activation of the HPG axis in in vitro-produced male dairy calves. FASEB J. 2021 Oct;35(10):e21882. doi:10.1096/fj.202101067R. PMID: 34460963.

320: Zhang Y, Chaput C, Fournier E, Prunier J, SIRARD MA. Comparing the whole genome methylation landscape of dairy calf blood cells revealed intergenerational inheritance of the maternal metabolism. Epigenetics. 2021 Jul 24:1-10. doi:10.1080/15592294.2021.1955188.

PMID:34304691.

319: Salilew-Wondim D, Tesfaye D, Rings F, Held-Hoelker E, Miskel D, SIRARD MA, Tholen E, Schellander K, Hoelker M. The global gene expression outline of the bovine blastocyst: reflector of environmental conditions and predictor of developmental capacity. BMC Genomics. 2021 Jun 3;22(1):408. doi: 10.1186/s12864-021-07693-0. PMID: 34082721; PMCID: PMC8176733.

318: Teplitz GM, Shi M, SIRARD MA, Lombardo DM. Coculture of porcine luteal cells during in vitro porcine oocyte maturation affects blastocyst gene expression and developmental potential. Theriogenology. 2021 Feb 25;166:124-134. doi: 10.1016/j.theriogenology.2021.02.014. Epub ahead of print. PMID: 33735666.

317: Shi M, SIRARD MA. Transcriptome and epigenome analysis of porcine embryos from non-esterified fatty acid-exposed oocytes. Domest Anim Endocrinol. 2021 Jan 16;76:106605. doi: 10.1016/j.domaniend.2021.106605. Epub ahead of print. PMID: 33631700.

316: Wu C, Blondin P, Vigneault C, Labrecque R, SIRARD MA. Sperm miRNAs- potential mediators of bull age and early embryo development. BMC Genomics. 2020 Nov 16;21(1):798. doi: 10.1186/s12864-020-07206-5. PMID: 33198638.

315: Wu C, SIRARD MA. Parental Effects on Epigenetic Programming in Gametes and Embryos of Dairy Cows. Front Genet. 2020 Oct 14;11:557846. doi:10.3389/fgene.2020.557846. PMID: 33173533; PMCID: PMC7591718.

314: Lafontaine S, Labrecque R, Palomino JM, Blondin P, SIRARD MA. Specific imprinted genes demethylation in association with oocyte donor’s age and culture conditions in bovine embryos assessed at day 7 and 12 post insemination. Theriogenology. 2020 Sep 24;158:321-330. doi:10.1016/j.theriogenology.2020.09.027. Epub ahead of print. PMID: 33010654.

313:  Manuel Palomino J, Grand FX, Vigneault C, Blondin P, SIRARD MA. Effects of follicular ablation and GnRH on synchronization of ovulation and conception rates in embryo recipient heifers. Anim Reprod Sci. 2020 Sep 14;221:106596. doi: 10.1016/j.anireprosci.2020.106596. Epub ahead of print. PMID: 32942212.

312 : de Lima CB, SIRARD MA. Mitoepigenetics: methylation of mitochondrial DNA is strand-biased in bovine oocytes and embryos. Reprod Domest Anim. 2020 Aug 1.doi: 10.1111/rda.13786. Epub ahead of print. PMID: 32738169.

311: Morin-Doré L, Blondin P, Vigneault C, Grand FX, Labrecque R, SIRARD MA. DNA methylation status of bovine blastocysts obtained from peripubertal oocyte donors. Mol Reprod Dev. 2020 Jul 17. doi: 10.1002/mrd.23399. Epub ahead of print. PMID: 32677283.

310: Fortin C, Hamilton S, Laforest M, Léveillé MC, SIRARD MA. Patients who failed to conceive following an IVF cycle can be clustered into different failure causes using gene expression hierarchical analysis. Biol Reprod. 2020 Jun doi: 10.1093/biolre/ioaa089. Epub ahead of print. PMID: 32483601.

309: Tremblay PG, SIRARD MA. Gene analysis of major signalling pathways regulated by gonadotropins in human ovarian granulosa tumour cells (KGN)† [published online ahead of print, 2020 May 19]. Biol Reprod. 2020 ;ioaa079. doi:10.1093/biolre/ioaa079

308: Lodde V, Garcia Barros R, Dall’Acqua PC, Dieci C, Robert C, Bastien A, SIRARD MA, Franciosi F, Luciano AM. Zinc supports transcription and improves meiotic competence of growing bovine oocytes. Reproduction. 2020May;159(6):679-691.PubMed PMID: 32191913.

307: Frasco, C.; Radmacher, M.; Lacroix, R.; Cue, R.; Valtchev, P.; Robert, C.; Boukadoum, M.; SIRARD MA. and Diallo, A. Towards an Effective Decision-making System based on Cow Profitability using Deep Learning.In Proceedings of the 12th International Conference on Agents and Artificial Intelligence – Volume 2: ICAART, ISBN 978-989-758-395-7, pages 949-958. (2020). DOI: 10.5220/0009174809490958

306: Bunel A, Nivet AL, Blondin P, Vigneault C, Richard FJ, SIRARD MA. The effects of LH inhibition with cetrorelix on cumulus cell gene expression during the luteal phase under ovarian coasting stimulation in cattle. Domest Anim Endocrinol. 2019 Dec 26;72:106429. doi: 10.1016/j.domaniend.2019.106429. [Epub ahead of print] PubMed PMID: 32320933.

305: SIRARD MA. Folliculogenesis and acquisition of oocyte competence in cows. Anim Reprod. 2019 Oct 23;16(3):449-454. doi: 10.21451/1984-3143-AR2019-0038. PMID: 32435288; PMCID: PMC7234107.

304: Chaput C, SIRARD MA. Embryonic response to high beta-hydroxybutyrate (BHB) levels in postpartum dairy cows. Domest Anim Endocrinol. 2019 Dec 31;72:106431. doi: 10.1016/j.domaniend.2019.106431. [Epub ahead of print] PubMed PMID: 32325411.

303: Wu C, Blondin P, Vigneault C, Labrecque R, SIRARD MA. The age of the bull influences the transcriptome and epigenome of blastocysts produced by IVF. Theriogenology. 2019 Dec 27;144:122-131. doi:10.1016/j.theriogenology.2019.12.020. [Epub ahead of print] PubMed PMID:31951983.

302: Caballero J, Blondin P, Vigneault C, SIRARD MA, Richard FJ. The use of adenosine to inhibit oocyte meiotic resumption in Bos taurus during pre-IVM and its potential to improve oocyte competence. Theriogenology. 2019 Oct 7;142:207-215. doi: 10.1016/j.theriogenology .2019.10.001. [PubMed PMID: 31614287.

301: SIRARD MA. Distribution and dynamics of mitochondrial DNA methylation in oocytes, embryos and granulosa cells. Sci Rep. 2019 Aug 15;9(1):11937. doi: 10.1038/s41598-019-48422-8. PubMed PMID: 31417147; PubMed Central PMCID: PMC6695495.

300: Fortin CS, Leader A, Mahutte N, Hamilton S, Léveillé MC, Villeneuve M, SIRARD MA. Gene expression analysis of follicular cells revealed inflammation as a potential IVF failure cause. J Assist Reprod Genet. 2019 Apr 18. doi: 10.1007/s10815-019-01447-4. PubMed PMID: 31001707.

299: Hue I, Dufort I, Vitorino Carvalho A, Laloe D, Peynot N, Degrelle S, Viebahn C, SIRARD MA. Different pre-implantation phenotypes of bovine blastocysts produced in vitro. Reproduction. 2018 Nov 1.doi:  10.1530/REP-18-0439. PubMed PMID: 30444718.

298: Tscherner A, Brown AC, Stalker L, Kao J, Dufort I, SIRARD MA, LaMarre J. STAT3 signaling stimulates miR-21 expression in bovine cumulus cells during in vitro oocyte maturation. Sci Rep. 2018 Aug 1;8(1):11527. doi:  10.1038/s41598-018-29874-w. PubMed PMID: 30068990.

297: 1: Landry DA, Rossi-Perazza L, Lafontaine S, SIRARD MA. Expression of atresia biomarkers in granulosa cells after ovarian stimulation in heifers. Reproduction. 2018 Jun 15. pii: REP-18-0186. doi: 10.1530/REP-18-0186. [Epub ahead of print] PubMed PMID: 29907662.

296: O’Doherty AM, McGettigan P, Irwin RE, Magee DA, Gagne D, Fournier E, Al-Naib A, SIRARD MA, Walsh CP, Robert C, Fair T. Intragenic sequences in the trophectoderm harbour the greatest proportion of methylation errors in day 17 bovine conceptuses generated using assisted reproductive technologies. BMC Genomics. 2018 Jun 5;19(1):438-448

295 Salilew-Wondim D, Saeed-Zidane M, Hoelker M, Gebremedhn S, Poirier M, Pandey HO, Tholen E, Neuhoff C, Held E, Besenfelder U, Havlicek V, Rings F, Fournier E, Gagné D, SIRARD MA, Robert C, Gad A, Schellander K, Tesfaye D. Genome-wide DNA methylation patterns of bovine blastocysts derived from in vivo embryos subjected to in vitro culture before, during or after embryonic genome activation. BMC Genomics. 2018 Jun 1;19(1):424.. PubMed PMID:29859035.

294: Laskowski D, Humblot P, SIRARD MA, Sjunnesson Y, Jhamat N, Båge R, Andersson G. DNA methylation pattern of bovine blastocysts associated to hyperinsulinemia in vitro. Mol Reprod Dev. 2018 May 10. PubMed PMID: 29745447.

293: Landry DA, SIRARD MA. Follicle capacitation: A meta-analysis to investigate the transcriptome dynamics following FSH decline in bovine granulosa cells. Biol  Reprod. 2018 Apr 14. [Epub ahead of print] PubMed PMID: 29668885.

292: Dias FCF, Khan MIR, SIRARD MA, Adams GP, Singh J. Transcriptome analysis of granulosa cells after conventional vs long FSH-induced superstimulation in cattle. BMC Genomics. 2018 Apr 16;19(1):258. PubMed PMID: 29661134.

291: SIRARD MA. 40 years of bovine IVF in the new genomic selection context.  Reproduction. 2018 Apr 10. pii: REP-18-0008. PubMed PMID: 29636405.

290: Laskowski D, Andersson G, Humblot P, SIRARD MA, Sjunnesson Y, Ferreira CR, Pirro V, Båge R. Lipid profile of bovine blastocysts exposed to insulin during in vitro oocyte maturation. Reprod Fertil Dev. 2018 Apr 16. [Epub ahead of print] PubMed PMID: 29655403.

289: Nivet AL, Dufort I, Gilbert I, SIRARD MA. Short-term effect of FSH on gene expression in bovine granulosa cells in vitro. Reprod Fertil Dev. 2018 Mar 13.  doi: 10.1071/RD17469. [Epub ahead of print] PubMed PMID: 29529392.

288: Vachon J, Pagé-Larivière F, SIRARD MA, Rodriguez MJ, Levallois P, Campagna C. Availability, quality and relevance of toxicogenomics data for human health risk assessment: A scoping review of the literature on trihalomethanes. Toxicol Sci.2018 Mar 5. doi:10.1093/toxsci/kfy050. [Epub ahead of print] PubMed PMID: 29514332.

287: SIRARD MA, Grand FX, Labrecque R, Vigneault C, Blondin P. The use of natural cycle’s follicular dynamic to improve oocyte quality in dairy cows and heifers. J Anim Sci. 2018 Mar 3. doi: 10.1093/jas/sky050. [Epub ahead of print] PubMed PMID:  29514310.

286: Ispada J, de Lima CB, SIRARD MA., Fontes PK, Nogueira MFG, Annes K, Milazzotto MP. Genome-wide screening of DNA methylation in bovine blastocysts with different kinetics of development. Epigenetics Chromatin. 2018 Jan 8;11(1):1. PubMed PMID: 29310712.

285: David A. Landry, Rémi Labrecque, François-Xavier Grand, Christian Vigneault, Patrick Blondin and SIRARD MAEffect of heifer age on the granulosa cell transcriptome after ovarian stimulation Reproduction, Fertility and Development 2018: 30 (7)

284:Tremblay R, Dufort I, SIRARD MA. Metabolic Stress Induces Modifications in the Epigenetic Program of Preimplantation Bovine Embryos. Mol Reprod Dev. 2017 Dec 14. [Epub ahead of print] PubMed PMID: 29240275.

283: Luciano AM, SIRARD MA. Successful in vitro maturation of oocytes: a matter of follicular differentiation. Biol Reprod. 2018 Feb 1;98(2):162-169. doi:

282: Lambert S, Blondin P, Vigneault C, Labrecque R, Dufort I, SIRARD MA. Spermatozoa DNA methylation patterns differ due to peripubertal age in bulls. Theriogenology. 2018 Jan 15;106:21-29.

PubMed PMID: 29031946.

281: Lodde V, Luciano AM, Franciosi F, Labrecque R, SIRARD MA. Accumulation of Chromatin Remodelling Enzyme and Histone Transcripts in Bovine Oocytes. Results Probl Cell Differ. 2017;63:223-255. PMID: 28779321.

280: Nivet AL, Vigneault C, Blondin P, SIRARD MA. Influence of luteinizing hormone support on granulosa cells transcriptome in cattle. Anim Sci J. 2018 Jan;89(1):21-30. PMID: 28840634.

Chaire de Recherche du Canada en Génomique de la Reproduction

http://www.reprogenomique.fsaa.ulaval.ca/