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Multiple fermenter system simulates the digestive conditions of the large intestine

The QPlus fermenter system (Sartorius) consists of three 0.5L fermenters directed by a control tower and BioPat software which analyzes all post-partum fermentation parameters. This system is mainly used to study the intestinal microbiota. We use the ImmoGut model, characterized by the immobilization of the intestinal microbiota into beads imitating the continuous liberation of bacteria from the intestinal wall to the intestinal tract.


Specifications

  • Simultaneous or serial fermentations
  • Completely independent control of the tank
  • Gassing system with controlled air flow
  • Integrated thermostat system
  • Maintenance-free agitation motor
  • Calibration by parallel probe function
  • Continuous pH control
  • Programming of time-bounded pH/temperature profile

Advantages of the ImmoGut model compared to other in vitro models

  • Environment with a high bacteria concentration
  • Possibility of conducting experiments over a long period of time
  • Simulation of continuous liberation of cells from the intestinal wall
  • Diversity and quantity of stable cells for the entire duration of the experiment
  • Use of the same intestinal microbiota for all samples, creating results that can be reproduced
  • Protection of cells from shearing force due to rotation in the fermenter

Even more benefits of using the multiple fermenter system service

  • Possibility of developing customized experiments (ex: short-chain amino acid dosage, quantification of live bacteria)
  • Simultaneous operation of the three fermenters to test numerous conditions and strains
  • Work in series to simulate three portions of the large intestine (proximal, transverse and distal)
  • BioPat software that analyzes post-fermentation results
  • Low-cost compared to testing on animals and humans

A. Photo of the bioreactor containing beads of immobilized intestinal cells
B. Photo taken with an electronic microscope showing the intestinal cells on a bead's surface Trends in Biotechnology January 2012, Vol. 30, No. 1