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Analytical services

Do you want to assay food or ingredient components?
Looking to characterize your product and highlight its properties?
Are you searching for a specific analysis that can’t be found in a private lab?


With our state-of-the-art techniques and sophisticated methods, INFA’s analytical laboratories make it easy to discover, characterize, and assay many different families of compounds. Our experts are able to analyze at least 150 different compounds such as vitamins, minerals, amino acids, antioxidants, polyphenols, flavonoids, anthocyanins, phytosterols, and others.

INAF’s analytical services can analyze and characterize your products. By determining their composition and highlighting their qualities we can help you promote your functional foods and ingredients.

Areas of expertise

Extraction

  • Development of conventional solvent and/or pressure based procedures using various plant matrices 
  • Development of extraction and purification processes, and enzymatic hydrolysis of metabolites for biological matrices (tissues, organs, urine, serum, plasma)

Assay

  • Liquid (HPLC, UPLC-MS/MS) or gas (GC) chromatography
  • Mass spectrometry (UPLC-MS/MS) for active compounds and their metabolites

Isolation and Identification

  • Purification of compounds using liquid, flash, and preparative countercurrent chromatography (CCC)
  • Identification by mass spectrometry (UPLC-MS/MS)
  • Identification by nuclear magnetic resonance (NMR) 

What we offer

Our studies and analyses are performed rapidly with quality as our top priority. Depending on your needs, we can also develop the analytical method, produce comprehensive analyses reports signed by a member of Ordre des chimistes du Québec, and provide support in interpreting results. Below is a list of some of the analyses we routinely supply.

 

Plant matrices

  • Antioxidant potential (ORAC, DPPH, Bates-Smith, BL-DMAC, European Pharmacopoeia)
  • Total polyphenols by spectrophotometry (Folin-Ciocalteu)
  • Polyphenol profiles by UPLC-MS (more than 50 different compounds)
  • Phytosterol profiles by GC
  • Organic acid profiles
  • Glucosinolate profiles
  • HPLC  profiles :
    • Anthocyanins
    • Proanthocyanidins
    • Carotenoids
    • Sugars

 Biological matrices (tissues, organs, urine, serum, plasma)

  • Polyphenols and their metabolites
  • Proanthocyanidins and their metabolites
  • Anthocyanins and their metabolites
  • Carotenoids

Analytical services team

Our team is composed of specialized professors, chemists, and highly qualified and experienced technicians trained in the chemistry of natural substances and biotechnology.

Facilities

Our analytical services laboratory has a large array of cutting-edge equipment and procedures, including:

  • Ultra high performance chromatography coupled with tandem mass spectrometry (UPLC-MS/MS)
  • Liquid chromatography (HPLC and UHPLC)
  • Gas chromatography (GC-FID and GC-MS)
  • Microplate readers (absorbance, fluorescence, luminescence)
  • Flash chromatography and preparative chromatography
  • Countercurrent chromatography
  • Fraction collector for sample fractionation
  • Lyophilizers and homogenizers
  • And more

Some completed projects

Additional references

Laurent Bazinet, Sylène Brianceau, Pascal Dubé, Yves Desjardins, Evolution of cranberry juice physico-chemical parameters during phenolic antioxidant enrichment by electrodialysis with filtration membrane, Separation and Purification Technology, Volume 87, 5 March 2012, Pages 31-39.

Chay Pak Ting, B.P.; Mine, Y.; Juneja, L.R.; Okubo, T.; Gauthier, S.F.; Pouliot, Y. Comparative Composition and Antioxidant Activity of Peptide Fractions Obtained by Ultrafiltration of Egg Yolk Protein Enzymatic Hydrolysates. Membranes 2011, 1, 149-161.

Mouna Ketata, Yves Desjardins, Cristina Ratti, Effect of liquid nitrogen pretreatments on osmotic dehydration of blueberries, Journal of Food Engineering, Available online 16 November 2012.